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By L. Badescu, D. Popescu

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Extra info for Algebraic Geometry, Bucharest 1982: Proceedings of the International Conference, Held in Bucharest, Romania, August 2-7, 1982

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In endometrium, the levels of E-cadherin and ·- and ß-catenin mRNA increased after ovulation, but reduced by the estrogen treatment, suggesting that the function of the adherens junction in the endometrium is regulated by sex steroids. Estrogen reduces cell-to-cell adhesive function and progesterone stimulates it. From the clinical aspect, the functions of the adherens junction to regulate the adhesive capacity in endometrial epithelial cells may be activated after ovulation. There are some related phenomena: estrogen stimulates the expression of E-cadherin in rat granulosa cells [15]; progesterone induces the expression of a cadherin-like protein, which leads to oocyte maturation in fertilized Xenopus oocyte [16].

As to the role of Fg and factor X III, previous authors, except those of our department, thought that it was only protection against bleeding during normal development of placenta. From our results, however, we came to believe firmly that Fg and factor X III not only worked as coagulation factors, but also worked with Fn as adhesive factors. Fg, Fn and factor X III are abundantly present in the endometrium also before implantation [pers. unpubl. data]. The subunits of ·5ß1 integrin (Fn receptor) have been shown to be present at the surface of cytotrophoblast [8] and type IV collagen to be present at the surface of decidual cell [9].

Negative controls were performed for all specimens. Serial sections were examined, and staining patterns were compared. , USA) twice, and then cut into small pieces. , USA) for 2 h at 37 ° C. Nondigestible tissues were removed with the Millipore Filter (n250 Ìm). Thereafter the cells were washed by centrifugation in HFM, containing 20% FCS. They were suspended in HFM, containing 10% FCS to a final concentration of 1 ! 105 cells/ml. 1 ! , USA), and cultured at 37 ° C in 5% CO2 and 95% air. We changed the medium every 48 h.

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